Effects of exogenous hormones treatment on spermiation and plasma levels of gonadal steroids in Roughscale sole, Clidoderma asperrimum

Roughscale sole (Clidoderma asperrimum) is only wild caught because basic reproductive research on this species lacking and gamete production in an artificial setting has not been successful. Exogenous hormone treatment used to induce gonadal maturation final spermiation wild-caught individuals. In study, the effects of exogenous roughscale was investigated by implanting different concentrations a salmon gonadotrophin-releasing analog (sGnRH; 0, 25, 50, 100 ?g/kg body weight) into male fishes. The control group did produce sperm after 21 days post-implantation, duration shorter compared other groups. period milt amount differed among hormone-treated groups according concentration used. Milt volumes treated with 25 sGnRH increased group, whereas had no effect movable ratio. spermatocrit high at beginning all then tended decrease gradually over time except experimental sGnRH. Plasma levels testosterone, 11-ketotestosterone, 17?, 20?-dihydroxy-4-pregnen-3-one were significantly affected treatments. Our results demonstrate that it possible prolong increase volume treating soles hormone. addition, affect motility.